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CRISPR/Cas9 CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats)is a DNA modification technique for target genes by RNA-guided Cas nucleases. Endonuclease Cas9 identifies specific genomic loci (PAM) by guide RNA (gRNA) and cleaves double-stranded DNA, resulti…
2020-02-13Guide RNA and Cas9 complex cause double-strand break (DSB) on the target site of DNA. Donor oligo carrying point mutation is the template for homologous recombination repair (HDR), and it recombines to the target site.
2020-02-13Guide RNA and Cas9 complex cause double-strand break (DSB) on the target site of DNA. Donor vector carrying knockin sequence is the template for homologous recombination repair (HDR), and it recombines to the target site.
2020-02-13The target coding sequence (CDS) was cloned into plasmid, and promoter on plasmid regulates the expression of the target gene. In addition, the reporter gene and selection marker can be used for screening. Ubigene developed a series of YOE plasmids, including lentivirus plasmids,…
2020-02-13RNA interference (RNAi) is induced by a highly conserved, double-stranded RNA (dsRNA), which would cause specific degradation of homologous mRNA. Specific genes can be silenced, resulting in the loss of function or suppression of gene expression.
2020-02-13